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PE0100 Sigma-Aldrich

phospho-ERK 1&2 (pThr185/pTyr187) ELISA

sufficient for 96 tests

Synonym: ERK 1&2 ELISA, Extracellular Signal Regulated Kinase, MAPK ELISA

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Description

Application

For research use only. Not for use in diagnostic procedures.
The assay is designed for the quantitative determination of human, mouse, and rat ERK1 dually phosphorylated at threonine 202 and tyrosine 204 and ERK2 dually phosphorylated at threonine 185 and tyrosine 187 in cell lysates.

Biochem/physiol Actions

ERK (Extracellular Signal Regulated Kinase), also known as MAPK (Mitogen-Activated Protein Kinase), has two isoforms. ERK1, also known as MAP kinase 1 or p44 MAP kinase, and ERK2, also known as MAP kinase 2 or p42 MAP kinase. These kinases belong to a family of serine/threonine kinases that are activated upon treatment of cells with mitogens, hormones, growth factors, cytokines and bioactive peptides. They are expressed broadly in normal tissues and various cell lines. Phosphorylation of ERK1&2 on a threonine and a tyrosine residues is required for enzyme activity of ERK1 and ERK2. Phosphorylation activates a signaling cascade, the downstream effects of which have been linked to the regulation of cell growth and differentiation as well as regulation of the cytoskeleton.

Features and Benefits

ERK 1&2 ELISAs offer a fast (total time 4 hours), sensitive (<16 pg/mL for non-phosphorylated and <0.8 units/mL for phosphorylated ERK 1&2), and specific (measures ERK 1&2 with no cross-reactivity from JNK and p38) alternative to immunoblotting or other bioassays.

Analysis Note

The sensitivity is <0.8 units/mL, which is 4 times more sensitive than immunoblotting. The assay is specific for ERK 1&2 with no cross-reactivity from JNK and p38. The data shows that only the phosphopeptide containing the phosphorylated threonine and tyrosine blocks the ELISA signal.

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9/PG 3
Protocols & Articles
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