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PE0230 Sigma-Aldrich

Plant Total Protein Extraction Kit

Suitable for any plant species or tissue

  •  NACRES NA.56

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Properties

Related Categories Molecular Biology, Plant Biotechnology, Plant Proteomics, Protein Extraction for Proteomics
storage temp.   −20°C

Description

General description

The Plant Total Protein Extraction Kit is designed specifically for use in plant bioscience to extract a qualitative sample of all proteins from any type of plant species or tissue. The protocol does not require any ultracentrifugation or aqueous polymer two-phase partitioning (APTP). The kit includes two reagents; a plant specific protease inhibitor cocktail and a new chaotropic reagent with increased solubilizing power to extract more hydrophobic proteins (C0356, Protein Extraction Reagent Type 4). Routine use of the reagent in sequential protein extraction of a plant tissue sample with fresh reagent removes nearly all protein from the tissue. The protease inhibitor cocktail is a mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, metalloproteases, aspartic, and aminopeptidases. It contains pepstatin A, 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, leupeptin, 1,10-phenanthroline, and bestatin. The cocktail has been demonstrated to be highly effective in preventing protein degradation during the extraction process.

Following removal of polyphenolics, tannins, and other interfering substances, ground plant tissue, fresh or frozen, is resuspended in the chaotropic reagent. Plant debris is pelleted by centrifugation and the protein extract is collected. The end result is a qualitative total protein sample, ready for downstream proteomic analysis.

Application

The plant total Protein Extraction Kit has been used to isolate crude seed protein. It has also been used to extract the total protein.

Quantity

Sufficient for preparation of 20 separate extractions from plant tissue samples of 10-250mg

Kit component also available separately

Description

Product #

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Protein Extraction Reagent Type 4 1 bottle SDS C0356
Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 1 mL SDS P9599
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3077 9 / PGIII
Flash Point(F) 
185 °F
Flash Point(C) 
85 °C

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the expected protein yield when using Product PE0230, Plant Protein Extraction Kit?
Depending on the tissue used (age, type (leaf, stem, root) health, etc.) one can expect 1-4 mg/mL at about 75-80% of the total protein.
How can I improve my yield when using Product PE0230, Plant Protein Extraction Kit?
A second extraction can be performed and combined with the first to increase protein extracted to about 95% of the total.
Will my protein samples be denatured when using Product PE0230, Plant Protein Extraction Kit?
Yes. The chaotropic agents and the detergent in the kit will denature the proteins at least partially.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles
Peer-Reviewed Papers
15

References

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