PHG0004 Sigma


PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

  • CAS Number 6976-37-0

  • Empirical Formula (Hill Notation) C8H19NO5

  • Molecular Weight 209.24

  •  Beilstein Registry Number 2205275

  •  EC Number 230-237-7

  •  MDL number MFCD00002853

  •  PubChem Substance ID 329823022



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
product line   PharmaGrade
form   powder
impurities   Endotoxin, microbial, and trace metals; tested
useful pH range   5.8 - 7.2
pKa (25 °C)   6.5
solubility   H2O: 0.1 M, clear, colorless
suitability   suitable for cell culture
  suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested



Bis Tris is a commonly used biological buffer for very broad usage in biological systems such as diagnostics, electrophoresis and protein based bioprocessing. The pKa of Bis Tris is 6.36 which makes it a good candidate for biological-based formulations.

Bis Tris is used in a wide variety of biological applications including protein separation buffers, cell culture media, electrophoresis separations and diagnostic reagent formulations.

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Enhanced Quality Product Documentation on PharmaGrade BIS-TRIS


1 kg in poly bottle

10, 25 kg in poly drum

100 g in poly bottle

Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles

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