BIS-TRIS propane

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane

  • CAS Number 64431-96-5

  • Linear Formula CH2[CH2NHC(CH2OH)3]2

  • Molecular Weight 282.33

  •  Beilstein/REAXYS Number 1786109

  •  EC Number 264-899-3

  •  MDL number MFCD00004689

  •  PubChem Substance ID 329823023

  •  NACRES NA.21



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   400
biological source   synthetic
Agency/Method   non-compendial
product line   PharmaGrade
form   powder
application(s)   cell culture | mammalian: suitable
impurities   Endotoxin, microbial, and trace metals; tested
useful pH range   6.3 - 9.5
pKa (25 °C)   (1) 6.8, (2) 9.0
mp   164-165 °C (lit.)
suitability   suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
InChI   1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2


General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


1 kg in poly bottle

10 kg in poly drum

100 g in poly bottle


Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.

A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.


Enhanced Quality Product Documentation on PharmaGrade BIS-TRIS Propane

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

pHast Pack
Protocols & Articles

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Enhanced Quality Program [VIDEO]

The Enhanced Quality Program is a quality management system that is used to differentiate products based on the required level of transparency, control and documentation support determined by the nee...

One Quality Policy [VIDEO]

Sigma-Aldrich maintains one quality policy on a global level to provide consistency in vision, maintained across all manufacturing and distribution sites so that the company on the whole is adhering ...

Quality Management Systems [VIDEO]

See how we combine the use of various Quality Management Systems to manage manufacturing and supply chain for our products, as well as ensure data integrity for information provided to customers via ...

Risk Management [VIDEO]

Our Quality Policy means that we are able to provide certain information to end customers based on a product’s Enhanced Quality level, and that information is limited to what we are able to guarantee...

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?