PHG0005 Sigma

BIS-TRIS propane

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane

  • CAS Number 64431-96-5

  • Linear Formula CH2[CH2NHC(CH2OH)3]2

  • Molecular Weight 282.33

  •  Beilstein Registry Number 1786109

  •  EC Number 264-899-3

  •  MDL number MFCD00004689

  •  PubChem Substance ID 329823023



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
product line   PharmaGrade
form   powder
impurities   Endotoxin, microbial, and trace metals; tested
useful pH range   6.3 - 9.5
pKa (25 °C)   (1) 6.8, (2) 9.0
mp   164-165 °C(lit.)
suitability   suitable for cell culture
  suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested



Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.

A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Enhanced Quality Product Documentation on PharmaGrade BIS-TRIS Propane


1 kg in poly bottle

10 kg in poly drum

100 g in poly bottle

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

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