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PHG0010 SAFC

Isopropyl β-D-1-thiogalactopyranoside

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production.

Synonym: IPTG, Isopropyl β-D-thiogalactoside

  • CAS Number 367-93-1

  • Empirical Formula (Hill Notation) C9H18O5S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 4631

  •  EC Number 206-703-0

  •  MDL number MFCD00063273

  •  PubChem Substance ID 329823027

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Properties

Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
InChI Key   BPHPUYQFMNQIOC-NXRLNHOXSA-N
product line   PharmaGrade
form   powder
impurities   residual solvents, tested
suitability   suitable for manufacturing use

Description

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.

Linkage

Enhanced Quality Product Documentation on PharmaGrade Isopropyl ß-D-1-thiogalactopyranoside (IPTG)

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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