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PIR00 Sigma-Aldrich

Inhibitor Resistant Genotyping PCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

Synonym: Inhibitor Resistant Genotyping PCR ReadyMix

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Properties

Related Categories Genomic DNA Amplification, Molecular Biology, PCR Inhibitor Resistant Readymixes, PCR/Amplification
form   liquid
feature   hotstart
storage condition   protect from light
color   light blue
shipped in   dry ice
storage temp.   −20°C

Description

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
• Bio-Rad® CFX384
• Bio-Rad CFX96
• Bio-Rad MiniOpticon
• Bio-Rad/MJ Chromo4
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon
• Cepheid SmartCycler®
• Eppendorf Mastercycler® ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene® 3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler® 480

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

LightCycler is a registered trademark of Roche

Mastercycler is a registered trademark of Eppendorf AG

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Rotor-Gene is a registered trademark of Qiagen GmbH

SmartCycler is a registered trademark of Cepheid, Inc.

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Inhibitor Resistant Genotyping Using Sigma's ReadyMixes™

Inhibitor Resistant Genotyping PCR ReadyMixes enable genetic analysis directly from crude samples such as lysates, blood spots, plant tissues and clinical samples. Each is a ready-to-use 2X concentra...
Keywords: Amplification, Clinical, Degradations, Genetic, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification

Protocols

Inhibitor Resistant Genotyping PCR ReadyMix™

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extract...
Keywords: Amplification, Cell disruption, Diagnostic, Environmental, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Titrations

Related Content

PCR Selection Guide

We offer a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from routine to enh...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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Add to Cart

PIR01 Inhibitor Resistant Genotyping PCR ReadyMix, Low ROX, with Low ROX for ABI and Stratagene instruments
PIR02 Inhibitor Resistant Genotyping PCR ReadyMix, ROX, with ROX for ABI instruments

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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