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PIR01 Sigma-Aldrich

Inhibitor Resistant Genotyping PCR ReadyMix, Low ROX

with Low ROX for ABI and Stratagene instruments

Synonym: Inhibitor Resistant Genotyping PCR ReadyMix

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Properties

Related Categories Genomic DNA Amplification, Molecular Biology, PCR Inhibitor Resistant Readymixes, PCR/Amplification
form   liquid
feature   hotstart
storage condition   protect from light
color   light blue
shipped in   dry ice
storage temp.   −20°C

Description

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. Inhibitor Resistant Genotyping PCR Readymix is compatible with both fast and conventional PCR cycling protocols.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:
• Applied Biosystems 7500
• Applied Biosystems 7500 Fast
• Applied Biosystems ViiA 7
• Stratagene Mx3000P®
• Stratagene Mx3005P®
• Stratagene Mx4000

Features and Benefits

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, ROX reference dye, insert blue qPCR dye, and stabilizers.

250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

Legal Information

Mx3000P is a registered trademark of Agilent Technologies, Inc.

Mx3005P is a registered trademark of Agilent Technologies, Inc.

Mx4000 is a trademark of Agilent Technologies, Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Inhibitor Resistant Genotyping Using Sigma's ReadyMixes™

Inhibitor Resistant Genotyping PCR ReadyMixes enable genetic analysis directly from crude samples such as lysates, blood spots, plant tissues and clinical samples. Each is a ready-to-use 2X concentra...
Keywords: Amplification, Clinical, Degradations, Genetic, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification

Protocols

Inhibitor Resistant Genotyping PCR ReadyMix™

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extract...
Keywords: Amplification, Cell disruption, Diagnostic, Environmental, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Titrations

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

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PIR02 Inhibitor Resistant Genotyping PCR ReadyMix, ROX, with ROX for ABI instruments
PIR00 Inhibitor Resistant Genotyping PCR ReadyMix, for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

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