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PLA0079 Sigma-Aldrich

Rabbit anti-PCNA Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym: ATLD2, DNA polymerase delta auxiliary protein, PCNA, cyclin



Related Categories Antibodies, Antibodies for Proximity Ligation Assays, Individual Antibodies More...
biological source   rabbit
application(s)   flow cytometry: 0.03 μg
  immunohistochemistry: 1:2,000- 1:10,000
  immunoprecipitation (IP): 2- 5 μg/mg
  western blot: 1:1,000- 1:10,000
species reactivity   human, mouse
shipped in   wet ice
storage temp.   2-8°C
antibody form   affinity purified immunoglobulin
antibody product type   primary antibodies
grade   Powered by Bethyl Laboratories, Inc.
accession no.   P12004
UniProt accession no.   P12004
Gene Information   rabbit ... PCNA(5111)



The epitope recognized by PLA0079 maps to a region between residues 75 and 125 of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).


Product PLA0079, Powered by Bethyl Laboratories, has been pre-qualified for use in proximity ligation assay (PLA®). PLA® technology enables visualization of protein interactions in fixed cells and tissue samples while under endogenous protein expression levels.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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