• USA Home
  • PLA0080 - Rabbit anti-PCNA Antibody, Affinity Purified

EMAIL THIS PAGE TO A FRIEND
PLA0080 Sigma-Aldrich

Rabbit anti-PCNA Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym: ATLD2, DNA polymerase delta auxiliary protein, PCNA, cyclin

Purchase

Properties

Related Categories Antibodies, Antibodies for Proximity Ligation Assays, Individual Antibodies More...
biological source   rabbit
application(s)   immunohistochemistry: 1:2,000-1:10,000
  immunoprecipitation (IP): 2- 5 μg/mg
  western blot: 1:5,000- 1:20,000
species reactivity   mouse, human
shipped in   wet ice
storage temp.   2-8°C
antibody form   affinity purified immunoglobulin
antibody product type   primary antibodies
grade   Powered by Bethyl Laboratories, Inc.
accession no.   P12004
UniProt accession no.   P12004
Gene Information   rabbit ... PCNA(5111)

Description

Immunogen

The epitope recognized by PLA0080 maps to a region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111).

Application

Product PLA0080, Powered by Bethyl Laboratories, has been pre-qualified for use in proximity ligation assay (PLA®). PLA® technology enables visualization of protein interactions in fixed cells and tissue samples while under endogenous protein expression levels.

Physical form

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?