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PLA0282 Sigma-Aldrich

Rabbit anti-Cyclin T1 Antibody, Affinity Purified

Powered by Bethyl Laboratories, Inc.

Synonym: CCNT, CDK9-associated C-type protein, CYCT1, Cyclin-T, HIVE1, cyclin C-related protein, human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1

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Properties

Related Categories Antibodies, Antibodies for Proximity Ligation Assays, Individual Antibodies More...
species reactivity   human
application(s)   immunohistochemistry: 1:100-1:500
  immunoprecipitation (IP): 2- 10 μg/mg
  proximity ligation assay: suitable
  western blot: 1:2,000- 1:10,000
antibody form   affinity purified immunoglobulin
grade   Powered by Bethyl Laboratories, Inc.
shipped in   wet ice
storage temp.   2-8°C
Gene Information   rabbit ... Cyclin T1(904)
biological source   rabbit
antibody product type   primary antibodies
accession no.   NP_001231.2

Description

Immunogen

The epitope recognized by PLA0282 maps to a region between residue 625 and 675 of human Cyclin T1 using the numbering given in entry NP_001231.2 (GeneID 904).

Physical form

Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide

Application

Product PLA0282, Powered by Bethyl Laboratories, has been pre-qualified for use in proximity ligation assay (PLA®). PLA® technology enables visualization of protein interactions in fixed cells and tissue samples while under endogenous protein expression levels.

Other Notes

Cyclin T1 is a regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, Cyclin T1 binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat′s affinity for the transactivating response RNA element (TAR RNA) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60563].

Legal Information

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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