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PLEX15 Sigma-Aldrich

GenElute Endotoxin-free Plasmid Maxiprep Kit

Green Alternative

sufficient for 15 purifications

Synonym: Gen Elute, GenElute Endotoxin-free Plasmid Kits

  •  NACRES NA.52

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Properties

Related Categories 12 Principles Aligned Products, Chemical Synthesis, DNA & RNA Purification, Greener Alternative Products, Greener Life Science Products,
Quality Level   200
usage   sufficient for 15 purifications
greener alternative product characteristics   Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.

Description

General description

The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines.

Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 250 μg of endotoxin-free plasmid DNA may be prepared from 5 to 40 mL of culture.

The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.

Application

The kit has been used to purify plasmid DNA for high efficiency transfection.

Features and Benefits

• High transfection efficiency and yield
• Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
• Fast and simple allowing up to 12 preps in less than 2 hours

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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