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PNS1010 Sigma

Stabilyser Reagent

for stabilization of DNA, RNA, and protein in tissue extracts

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Properties

form   liquid
feature   for stabilization of DNA, RNA, and protein in tissue extracts
shipped in   wet ice
storage temp.   2-8°C

Description

Application

Prevents degradation of proteins and nucleic acid during tissue lysis and storage. Stabilize functional proteins and RNA.

Features and Benefits

• Gentle, non-denaturing lysis buffer does not interfere with downstream applications
• Convenient ready-to-use reagent
• Stabilizes RNA and protein for analysis at a later date

General description

The stability of protein and antibody structure and function is dependent on a variety of factors such as temperature, freeze/thaw cycles, proteases and pH. For some proteins and antibodies, function can be lost completely with a single freeze/thaw cycle and proteases can significantly degrade proteins and antibodies within hours even when kept at 4 °C. Stabilyser Reagent minimizes the risk to improve protein assay quality and accuracy.

Stabilyser Reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization and storage of protein, DNA, and RNA from tissue samples. The unique composition of Stabilyser enables isolation of functionally active proteins, while maintaining integrity of nucleic acids. Stabilization of proteins and RNA is achieved even after 24 hours at room temperature Stabilyser reagent is compatible with Western blotting protocols, functional protein activity assays, and nucleic acid applications like qPCR.

Use in conjunction with a homognenizer for simultaneous tissue lysis and stabilization of protein and nucleic acids in tissue samples.

Preparation Note

Ready-to-use reagent. In general it is recommended to start optimization with 20 μL of Stabilyser reagent per mg of tissue.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

RNA stabilization and protein stabilization in tissue extracts with Stabilyser™ Reagent

Maintain nucleic acids and functional proteins with Stabilyser™ reagent, the only reagent that protects the integrity of nucleic acid and active protein in one uniform lysate mixture. Now, you can st...
Keywords: Cell disruption, Enzyme activity, Homogenization, Polymerase chain reaction - quantitative, Western blot

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