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PP0510 Sigma-Aldrich

GlycoProfile IV Chemical Deglycosylation Kit

TFMS Deglycosylation System

  •  NACRES NA.32

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Description

Application

The GlycoProfile IV Kit utilizes trifluoromethanesulfonic acid (TFMS) in a deglycosylation system that completely removes all N- and O-linked glycans while preserving the protein/polypeptide structure. TFMS hydrolysis of glycoproteins results in minimal protein degradation while the released glycans are destroyed. For high molecular weight or complex, non-mammalian glycoproteins, an optional scavenger species is included. Complete deglycosylation of glycoproteins takes place in as little as 30 minutes.

Quantity

The kit contains sufficient reagents for 10 reactions with a sample size between 1-2 mg of a typical glycoprotein. An RNase B glycoprotein standard is included.

Legal Information

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Kit component only

Description

Product #

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Trifluoromethanesulfonic Acid (TFMS) 5 x 1 g    
RNase B Glycoprotein Standard 3 x 1 mg    
Pyridine solution, 60% 10 mL    
Bromophenol blue solution, 0.2% .5 mL    
Anisole, anhydrous 5 x 1 mL    
Reaction vials 10 ea    
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3316 9
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Glycoprotein Deglycosylation

Aparagine-linked (N-linked) and serine/threonine-linked (O-linked) oligosaccharides are major structural components of many eukaryotic proteins. They perform critical biological functions in protein ...
BioFiles 2007, 2.1, 7.
Keywords: Degradations, Dialysis, Digestions, Filtration, Glycosylations, High performance liquid chromatography, Inflammation, Ligands, Mass spectrometry, Methods, PAGE, Proteomics, Substitutions, Type

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