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PROTTOT Millipore

ProteoPrep® Total Extraction Sample Kit




This kit provides four extraction reagents of increasing solubilizing power. Each can generate total protein extracts from cellular samples. Along with conventional reagents, the kit also includes the newest generation of detergent reagents. This allows comparison of the protein extractions obtained with each of the four reagents and optimization to meet your individual needs. The special reducing and alkylating reagents produce protein samples that exhibit improved focusing and decreased streaking in 2D gels. The ProteoPrep Total Extraction Kit was designed through a collaboration of Proteome Systems and Sigma research scientists.
For researchers who have optimized an extraction protocol using one chaotropic extraction reagent, each reagent is available as an individual product as well.

Features and Benefits

• Four pre-mixed solubilization reagents - Enables rapid solubilization.
• Pre-measured reducing and alkylating reagents - Easy-to-use reagents provide improved IEF resolution.
• Innovative detergent preparations - Highly improved solubility allows higher protein loads and greater visibility of low abundance proteins in 2D gels.

Legal Information

ProteoPrep is a registered trademark of Sigma-Aldrich Co. LLC

Kit component also available separately


Product #

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Protein Extraction Reagent Type 1 SDS C0481
Protein Extraction Reagent Type 2 SDS C0606
Protein Extraction Reagent Type 3 SDS C0731
Protein Extraction Reagent Type 4 SDS C0356
Iodoacetamide, Single use vial of 56 mg 5 x 56 mg SDS A3221
Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5 mL SDS T7567
Safety & Documentation
Protocols & Articles
Peer-Reviewed Papers


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