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QR0100 Sigma-Aldrich

SYBR® Green Quantitative RT-qPCR Kit

One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

  •  NACRES NA.55

Purchase

Description

Application

SYBR®Green Quantitative RT-qPCR Kit has been used in Real-time RT-PCR. It has also been used for CHIKV (Chikungunya virus) viral RNA quantification by using One-step SYBR green-based RT-PCR.

The SYBR Green Quantitative RT-PCR kit provides a highly sensitive method for the quantitative analysis of gene expression. Sigma′s QRT-PCR ReadyMix combines the advantages of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart Taq in a convenient ReadyMix.

Packaging

1 kit sufficient for 100 reactions at 50 μL each

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

SYBR is a registered trademark of Life Technologies

Kit component only

Description

Product #

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SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25 mL    
Molony Murine Leukemia Virus Reverse Transcriptase (M-MLV™) 5000 U    

Standard component

Description

Product #

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10X PCR Buffer, Optimized for routine PCR with MgCl2 included SDS P2192
25 mM MgCl2 1.5 mL/vial SDS M8787
Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vial SDS R4526
Safety & Documentation

Safety Information

RIDADR 
UN 3316 9
Protocols & Articles

Articles

Metagenomics and Quantitative PCR

The highly variable regions within the 16S ribosomal RNA (rRNA) gene sequences are being exploited with PCR and metagenomic sequencing to characterize the microbial strains within the gastrointestina...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Gastrointestinal, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Probiotics, Sequencing

Reverse Transcription - PCR Technologies Guide

Use the table of contents on the right to navigate to other sections of A Technical Guide to PCR Technologies.
A Technical Guide to PCR Technologies
Keywords: Degradations, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

Protocols

Primer Concentration Optimization Protocol

Optimization of qPCR conditions is important for the development of a robust assay. Indications of poor optimization are a lack of reproducibility between replicates as well as inefficient and insens...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)

In the example given below, the primer concentrations can be adjusted according to the results of optimization procedures (see Primer Concentration Optimization, Primer Optimization Using Temperature...
Keywords: Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Related Content

Automated Protocols for Life Science Research

Our scientists have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply information such as i...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Master Mix

Sometimes known as super mix or ready mix, PCR master mix is a batch mixture of PCR reagents at optimal concentrations that can be prepared once and divided among many PCR tubes or 96-well PCR plates...
Keywords: Buffers, Gene expression, Nucleic acid hybridization, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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