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R1155 Sigma-Aldrich

Anti-AtRabA4 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-AtGB3, Anti-AtRab11G, Anti-AtRabA4b

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1-2 μg/mL using whole cell extract of E. coli expressing GST-AtRabA4b fusion protein
species reactivity   Arabidopsis thaliana
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
Gene Information   Arabidopsis thaliana ... RABA4B(830160)

Description

General description

The Arabidopsis thaliana Rab GTPase (RabA4b) is a Rab GTPase, that is expressed in various plant organs. In A. thaliana, RabA4b is ubiquitously expressed, mainly located at an inverted cone-shaped area in the apical region in the root hairs. The Arabidopsis genome contains a total of 93 small GTPase genes, including 57 members of the Rab family. Arabidopsis thaliana Rab GTPase RabA4b (AtRabA4b, AtGB3, AtRab11G) is highly similar to AtRab11.

Specificity

Anti-AtRabA4bspecifically recognizes recombinant AtRabA4b fusion protein.

Immunogen

synthetic peptide corresponding to amino acids 201-219 located near the C-terminus of Arabidopsis thaliana AtRabA4b fusion protein.

Application

Anti-AtRabA4 antibody produced in rabbit may be used in immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The Arabidopsis thaliana Rab GTPase (RabA4b) can control membrane trafficking steps involved in the polarized deposition of cell wall components in tip-growing root hair cells. AtRabA4b can label the trans-Golgi network compartment displaying a developmentally regulated polar distribution in growing root hair cells of A. thaliana.

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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