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R125 Sigma-Aldrich

Anti-RANTES antibody produced in goat

affinity isolated antibody, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   immunohistochemistry (frozen sections): suitable
  neutralization: suitable
  western blot: suitable
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P13501
Gene Information   human ... CCL5(6352)

Description

General description

RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted; CCL5) is a chemokine that brings T cells, monocytes, eosinophils, NK cells, dendritic cells and mast cells to the site of infection or inflammation. RANTES has an unusual kinetic profile and is secreted 3-5 days after the activation of the T cells. It also exhibits antimicrobial activity by inducing the production of nitric oxide in macrophages. The main function of RANTES is mediated by CCR5, CCR1, CCR3 and other receptors. The expression of RANTES is regulated mainly by JAK/STAT and NF-κB pathways. Polymorphisms in the gene encoding RANTES are implicated in artherosclerosis, multiple sclerosis, HIV infection, diabetes, inflammatory diseases of the lung, renal disease and systemic lupus erythematosus. In addition, RANTES is a potential prognostic factor to track the progression of stage II breast cancer
Anti-RANTES antibody specifically recognizes human RANTES. The antibody shows ~40% cross-reactivity with mouse RANTES and canine RANTES, ~20% cross-reactivity with cotton rat RANTES, and ~1% cross-reactivity with feline RANTES.

Specificity

Based on ELISA, the antibody shows ~40% cross-reactivity with recombinant mouse RANTES, ~20% cross-reactivity with recombinant cotton rat RANTES, and ~1% cross-reactivity with recombinant feline RANTES.

Immunogen

recombinant human RANTES expressed in E. coli.

Application

Anti-RANTES antibody may be used for immunoblotting at a working concentration of 0.1 μg/ml. For immunohistochemistry and immunofluorescence, a working concentration of 5-15 μg/ml may be used. The antibody is suitable for neutralization reactions (ND50 is 0.1-0.4 μg/ml).

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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