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R1780 SAFC

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications

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Properties

Related Categories Cell Culture, Cell Culture Media, Classical Media and Salts, Core Bioreagents, Life Science Reagents for Cell Culture,
Quality Level   500
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
components   glucose: 2 g/L (Dextro)
L-glutamine: 0.3 g/L
NaHCO3: 2 g/L
phenol red: no
shipped in   ambient

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Reconstitution

Designed for use in stable isotope labeling with amino acids in cell culture applications.
Supplement with 0.100 g/L L-Arginine-13C6, 15N4 HCl (Product 608033), 0.100 g/L L-Leucine-13C6, 15N (Product 608068), and 0.100 g/L L-Lysine-13C6, 15N2 (Product 608041).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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