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R2405 Sigma-Aldrich

RPMI-1640 Medium

AQmedia, With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories AQMedia, Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media,
Quality Level   PREMIUM
grade   AQmedia
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   NaHCO3: yes
sodium pyruvate: no
HEPES: no
stable glutamine: yes
phenol red: yes
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Other Notes

This RPMI-1640 medium is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

Legal Information

AQmedia is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

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