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  • R2650 - Monoclonal Anti-Rac antibody produced in mouse

R2650 Sigma

Monoclonal Anti-Rac antibody produced in mouse

clone 23A8, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   rat, mouse, human
application(s)   immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  western blot: 0.5-2.0 μg/mL using rat brain microsomal protein preparation
clone   23A8, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG
mol wt   antigen mol wt 21 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ARHGEF6(9459)
mouse ... Arhgef6(73341)
rat ... Arhgef6(363509)
biological source   mouse
conjugate   unconjugated



recombinant protein containing the full length human Rac1.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide with 30% glycerol.


The antibody recognizes Rac1 with a slight Rac2 cross-reactivity.

Preparation Note

Purified using protein G

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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