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  • R2775 - Monoclonal Anti-RasGAP antibody produced in mouse

R2775 Sigma

Monoclonal Anti-RasGAP antibody produced in mouse

clone B4F8, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Monoclonal Anti-Gap, Monoclonal Anti-Ras GTPase-activating protein, Monoclonal Anti-Rasa1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   mouse, bovine, rat, human, hamster
application(s)   immunocytochemistry: 10 μg/mL using A431 cells fixed with ethanol:acetic acid (95:5)
  immunoprecipitation (IP): 2.5 μg using 500 μg of A431 cell lysate
  western blot: 0.5-2 μg/mL using 20 μg of A431 cell lysate
clone   B4F8, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous glycerol solution
isotype   IgG
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... RASA1(5921)
mouse ... Rasa1(218397)
rat ... Rasa1(25676)
biological source   mouse
conjugate   unconjugated



full-length recombinant human GAP expressed in Sf9 insect cells.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.4, containing 0.105 M sodium chloride, 30% glycerol, and 0.035% sodium azide.

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Certificate of Analysis

Certificate of Origin

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WGK Germany  1
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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