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  • R3025 - Anti-Rho-GDI antibody produced in rabbit

R3025 Sigma

Anti-Rho-GDI antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   bovine, mouse, human
application(s)   immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate
  western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31
clone   polyclonal
concentration   ~1 mg/mL
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~28 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... ARHGDIA(396)
mouse ... Arhgdia(192662)
biological source   rabbit
conjugate   unconjugated



GST fusion protein corresponding to full-length human Rho-GDI.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.


The antibody recognizes Rho-GDI and cross-reacts with D4-GDI.

Preparation Note

Purified using protein A

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  1
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Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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