• USA Home
  • R3150 - Monoclonal Anti-RhoGAP (p190) antibody produced in mouse

R3150 Sigma

Monoclonal Anti-RhoGAP (p190) antibody produced in mouse

clone D2D6, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-GRLF1, Anti-Glucorticoid Receptor Binding Factor 1, Anti-p190



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to G Proteins, Antibodies to G Proteins and Cyclic Nucleotides,
species reactivity   mouse, monkey, human, rat
application(s)   immunoprecipitation (IP): 4 μg using 500 μg of a human A431 cell RIPA lysate
  western blot: 0.5-2 μg/mL using RIPA lysates from human A431 cells
clone   D2D6, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG
mol wt   antigen mol wt ~190 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... GRLF1(2909)
mouse ... Grlf1(232906)
rat ... Grlf1(306400)
biological source   mouse
conjugate   unconjugated



GST fusion protein corresponding to amino acids 180-610 of rat p190 RhoGAP.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 sodium chloride and 0.05% sodium azide.

Preparation Note

Purified using protein G.

Other Notes

RhoGAP appears to couple signal transduction via Ras and Rho through its association with RasGAP.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?