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R3884 Sigma-Aldrich

EclX I from Enterobacter cloacae 590

Restriction Enzyme

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Specificity

Recognition sequence: 5′-C/GGCCG-3′
Ligation and recutting results: After 2-10-fold EclX I overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >95% of fragments can be ligated, and >95% recut.
Heat inactivation: 65 °C for 20 minutes.

Application

EclXI is a DNA restricition enzyme used in molecular biology applications to cleave DNA at the recognition site 5′-C/GGCCG-3′ to generate fragments with 5′-cohesive ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SB (B8781).

Linkage

Isoschizomer: Xma III

Physical form

Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 100 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v),100 μg/ml BSA, 0.05% polydocanol, 100 mg/ml hydroxy-ectione at 4°C

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Our restriction enzyme collection has been optimized for digestion using five unique buffers. Reference the table below for the enzyme activity in each buffer.
Keywords: Buffers, Digestions, Enzyme activity, Solid phase extractions

Protocols

Restriction Enzyme Digest Protocol

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also identified on tab...
Keywords: Buffers, Digestions, Purification

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Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
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Peer-Reviewed Papers
15

References

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B8781 Restriction Enzyme Buffer SB, 10 ×

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