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R4526 Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

Synonym: ROX

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Properties

Related Categories Molecular Biology, PCR/Amplification, Quantitative PCR, Reagents, Traditional QPCR More...
form   solution
usage   sufficient for ≥600 reactions
concentration   100 ×
color   red
storage temp.   2-8°C

Description

Application

Reference Dye for Quantitative PCR has been used in the preparation of mastermix RT-qPCR (reverse transcriptase-quantitative polymerase chain reaction).

Sigma′s Reference Dye for qPCR is a proprietary dye for use with real-time PCR. It is used for normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

Packaging

0.3 mL in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Hot Start Amplification of DNA with SYBR® Green JumpStart™ Taq ReadyMix™

The single most important step in assuring success with PCR is high quality DNA preparation. Integrity and purity of DNA template is essential. Quantitative PCR involves multiple rounds of enzymatic ...
Keywords: AGE, Amplification, Diagnostic, Electrophoresis, Gas chromatography, Gel electrophoresis, Gene expression, Melting, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Separation, Size-exclusion chromatography, Solvents

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

Related Content

PCR Selection Guide

We offer a wide variety of PCR enzymes, master mixes, and PCR protocols to meet your experimental needs for routine PCR, qPCR, or RT-PCR. Our PCR Selection Guide features various filters to sort by, ...
Keywords: Genomics, Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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