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R5125 Sigma

Ribonuclease A from bovine pancreas

Type III-A, ≥85% RNase A basis (SDS-PAGE), 85-140 Kunitz units/mg protein

Synonym: Pancreatic Ribonuclease, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase



Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Cell Biology,
Quality Level   PREMIUM
type   Type III-A
assay   ≥85% RNase A basis (SDS-PAGE)
form   lyophilized powder
mol wt   mol wt ~13,700
impurities   salt, essentially free
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   protease, essentially free
storage temp.   −20°C


Analysis Note

Protein determined by E1%/280.

RNase A purity determined by SDS-PAGE

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

General description

Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.


1 g in glass bottle

25, 50, 100, 250, 500 mg in glass bottle

Preparation Note

A chromatographic purification of R5503


Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess nickel-dependent oxidative cross-linking of a protein. Ribonuclease A from bovine pancreas has also been used in a study to investigate equilibrium constants for nonspecific binding of proteins to DNA.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Keywords: Chromatography, High performance liquid chromatography


Enzymatic Assay of Ribonuclease A (EC

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

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Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
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Peer-Reviewed Papers


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