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R5628 Sigma-Aldrich

Hae III from Haemophilus aegyptius

Restriction Enzyme

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Enzyme Class Index, Enzymes, Inhibitors, and Substrates,
grade   for molecular biology
form   buffered aqueous glycerol solution
concentration   10,000 units/mL
shipped in   wet ice
storage temp.   −20°C

Description

Specificity

Recognition sequence: 5′-GG/CC-3′
Ligation and recutting results: After 2-10-fold Hae III overdigestion of 1 μg λ DNA substrate, results in 100% cutting, >50% of fragments can be ligated, and >95% recut.
Heat inactivation: 80 °C for 20 minutes.

Application

HaeIII is a restriction enzyme that is used in molecular biology methods to cleave DNA at the recognition site 5′-GG/CC-3′ to generate DNA fragments with blunt termini.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SM (B3158).

Comment: Inefficient for single-stranded DNA cleavage.
Hae III requires optimal reaction conditions in order to avoid star activity.

Physical form

Solution in 20 mM Tris-HCl, pH 7.7, 0.1 mM EDTA, 400 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v) at 4°C

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Our restriction enzyme collection has been optimized for digestion using five unique buffers. Reference the table below for the enzyme activity in each buffer.
Keywords: Buffers, Digestions, Enzyme activity, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Our restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also identified on tab...
Keywords: Buffers, Digestions, Purification

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

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