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R5636 Sigma-Aldrich

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

buffered aqueous solution

Synonym: Transfer RNA, tRNA

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Properties

Related Categories Carrier Molecules, DNA & RNA Purification, Molecular Biology, Reagents
grade   for molecular biology
form   buffered aqueous solution
concentration   ≥9 mg/mL
foreign activity   DNase, Nickase, none detected
shipped in   dry ice
storage temp.   −20°C

Description

General description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Application

Suitable for use as a carrier in nucleic acid purification and precipitation.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:
• in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assay
• as a component of prehybridization(4) and hybridization buffer in single-label in situ hybridization
• as a carrier to enhance recovery of RNA from small numbers of cells

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.

Components

tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.


Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Ribonucleic acid, transfer from baker's yeast (S. cerevisiae)

Product Description Precautions and Disclaimer Storage/Stability Procedure Materials References
Keywords: AGE, Electrophoresis, Gel electrophoresis, Precipitation

Peer-Reviewed Papers
15

References

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