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RPMI-1640 Medium

HEPES Modification, With 25 mM HEPES, without L-glutamine., liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
Quality Level   300
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
pH range   >7.2
components   L-glutamine: no
NaHCO3: yes
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Other Notes

This RPMI-1640 medium is supplemented with HEPES. HEPES is a Good’s physiological buffer that helps provide a more stable medium pH than bicarbonate buffers.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

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Peer-Reviewed Papers
15

References

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