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  • R6028 - Anti-ROCK-1 antibody produced in rabbit

R6028 Sigma-Aldrich

Anti-ROCK-1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Rho-kinase



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 10-20 μL using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
  microarray: suitable
  western blot: 1:1,000-1:2,000 using of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line
species reactivity   human, rat, mouse
mol wt   antigen 160 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   Q13464
Gene Information   human ... ROCK1(6093)
mouse ... Rock1(19877)
rat ... Rock1(81762)


General description

Rho-associated coiled-coil protein kinase 1 or ROCK-1 is a serine/threonine kinase that regulates actin assembly, cell movement and contraction. This kinase activates the JNK signaling pathway, modulates stress granule formation and regulates apoptosis in cardiac myocytes and stress granules .


synthetic peptide corresponding to amino acids 1336-1354 located at the C-terminus of human ROCK-1, conjugated to KLH. This sequence is identical in mouse and rat ROCK-1.


Anti-ROCK-1 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Rho-associated kinases (ROCK or Rho-kinase) is implicated in Rho-mediated actin reorganization such as formation of stress fibres and focal adhesions and smooth muscle contraction. Rho-kinases lead to an increase of myosin light chain (MLC) phosphorylation and thereby induce actomyosin based contractility. ROCK accumulates at the cleavage furrow during cytokinesis. In addition, a dominant negative form of ROCK increases multinuclei in mammalian cells. ROCK directly phosphorylates and activates LIM-kinase (LIMK), resulting in downstream phosphorylation and inaction of the actin depolymerizing factor or cofilin.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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