• USA Home
  • R6513 - Ribonuclease A from bovine pancreas

EMAIL THIS PAGE TO A FRIEND
R6513 Sigma-Aldrich

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

Synonym: Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

Purchase

Description

General description

RNase A is a single chain polypeptide containing 124 amino acids, linked by four disulfide bridges. In contrast to RNase B, RNase A is not a glycoprotein. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.

RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Application

• RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
• RNase A is also used in RNA sequence analysis and protection assays.
• RNase A has been used as a tool for computer-aided drug design.
• RNase A supports the analysis of RNA sequences.
• RNase A hydrolyze RNA contained in protein samples.
• Purification of DNA is supported by RNase A.

Ribonuclease A from bovine pancreas has been used:
• for plasmid DNA purification
• to digest and avoid staining of double-stranded RNA during staining of nuclei
• in immunoperoxidase and immunofluorescence detection
• to treat cells prior to flow cytometry
• as a component of post-hybridization buffer for washing FFPE tissue sections post in-situ hybridization
• to treat cells during the preparation of outer membrane protein (OMP) extracts
• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

Analysis Note

Protein determined by E.

Other Notes

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 μg/mL.

Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.

Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
RF0760000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the solubility of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
Stock solutions of RNase A can be made in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl at a concentration of 10 mg/mL.
What is the shelf life of Product R6513, Ribonuclease A from bovine pancreas (RNase A)?
This product is stable for at least 3 years when stored properly at -20 °C.
How are solutions of Product R6513, Ribonuclease A from bovine pancreas (RNase A), stored?
RNase A solutions can be stored, aliquoted and frozen at -20 °C.
Do I need to boilProduct R6513, Ribonuclease A from bovine pancreas (RNase A), to inactivate residual DNases?
Boiling of stock solutions to inactivate residual DNase is not necessary and may cause precipitation of RNase with loss of enzymatic activity.
What working concentration of Product R6513, Ribonuclease A from bovine pancreas (RNase A), do you suggest?
We suggest using RNase A at a concentration of 10 ug/ml.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Why is RNase A activity not listed as micromoles per minute, and what is a Kunitz unit?
The Kunitz unit was created to reflect the non-linear nature of the RNAse enzymatic reaction. As the reaction progresses the activity changes due to RNA as a heterogeneous polymeric substrate; therefore, the unit of moles or micomoles per minute becomes inaccurate. As such, our enzymatic assay describes a unit that varies throughout the reaction: One unit will cause a decrease of 100% per minute in the value of E0 - Ef at pH 5.0 at 25°C. Ef is determined in the RNAse control step, while E0 reflects the current reaction rate.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Low Moleculer Weight Proteins on 5μm BIOshell™ 160Å Peptide C18 Column, Comparison to Totally Porous 3 μm C18 Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Peptide Mixture on Zenix® SEC-100

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptide Mixture on Zenix® SEC-100
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 3 cm x 4.6 mm I.D. Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Peptides and Proteins on Proteomix® SCX-NP1.7, 3 cm x 4.6 mm I.D. Column
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® SCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Comparing Ion Selectivity on Proteomix® WCX-NP5
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins Demonstrating Selectivity Differences between TSKgel® Strong (SP-5PW) and Weak (CM-5PW) Cation Exchangers
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX, Comparison of Different Particle Sizes
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins and Peptides on Proteomix® WCX-NP1.7 Demonstrating Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on BIOshell™ A160 Peptide C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP1.7, Demonstration of Lot-to-Lot Reproducibility
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Proteomix® SCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® SCX-NP5
Keywords: High performance liquid chromatography, Separation

HPLC Analysis of Proteins on Proteomix® WCX-NP5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Proteomix® WCX-NP5
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison
Keywords: Chromatography, High performance liquid chromatography, Ion Exchange

HPLC Analysis of Proteins on TSKgel® BioAssist S

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® BioAssist S
Keywords: High performance liquid chromatography, Proteomics

HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on TSKgel® Porous (Phenyl-5PW and Ether-5PW) and Non-Porous (Butyl-NPR) HIC Columns
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm
Keywords: Chromatography, High performance liquid chromatography, Separation, Size-exclusion chromatography

HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins with Different pI Values on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
Keywords: Chromatography, Clinical, High performance liquid chromatography, Size-exclusion chromatography

Loading Capacity of RNaseA on Proteomix® SCX-NP3

From our library of Articles, Sigma-Aldrich presents Loading Capacity of RNaseA on Proteomix® SCX-NP3
Keywords: Chromatography, High performance liquid chromatography

Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins

From our library of Articles, Sigma-Aldrich presents Scalability of TSKgel® Phenyl-5PW Analytical HPLC Separations to Preparative/Process Scale on Toyopearl® Phenyl-650M Resins
Keywords: Chromatography, High performance liquid chromatography

Protocols

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

Related Content

Enzymes & Proteins

Committed to developing and providing breakthrough problem-solving reagents and solutions, explore our comprehensive offering of enzymes and protein reagents and kits with the confidence that you wil...
Keywords: Cell signaling, Diagnostic, Enzyme-linked immunosorbent assay, Immunohistochemistry, Metabolism, Molecular biology, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

inhibitor

Product #

Description

Add to Cart

D5758 Diethyl pyrocarbonate, ≥97% (NMR)
R4642 Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
R2520 Ribonuclease inhibitor human, Inhibits ribonucleases during transcription and translation experiments

related product

Product #

Description

Add to Cart

LIG1 DNA Ligation Kit
D8276 DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution
P9380 Pyridoxamine dihydrochloride, analytical standard, ≥98%
D2886 DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution
P4978 Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C
P4390 Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution
G5663 Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
D7291 Deoxyribonuclease I RNase-free solution from bovine pancreas

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?