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  • R6878 - Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse

R6878 Sigma

Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-phospho-Rb (pSer795)



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human, hamster, mouse, bovine, rat
application(s)   immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
  indirect ELISA: suitable
  microarray: suitable
  western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361
clone   RB-10, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 110 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)
biological source   mouse
conjugate   unconjugated



synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer795) of human retinoblastoma protein conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

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WGK Germany  2
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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