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R7017 Sigma-Aldrich

Resazurin sodium salt

powder, BioReagent, suitable for cell culture

Synonym: 7-Hydroxy-3H-phenoxazin-3-one-10-oxide sodium salt

  • CAS Number 62758-13-8

  • Empirical Formula (Hill Notation) C12H6NNaO4

  • Molecular Weight 251.17

  •  Beilstein/REAXYS Number 3794971

  •  EC Number 263-718-5

  •  MDL number MFCD00005036

  •  PubChem Substance ID 24899387

  •  NACRES NA.75

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Properties

Related Categories Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Base Ingredients for Media, Cell Culture, Indicators, Microbiology,
Quality Level   200
product line   BioReagent
form   powder
composition   Dye content, ~80%
application(s)   cell culture | mammalian: suitable
pH range   3.8 - 6.5, orange to violet
solubility   H2O: soluble
λmax   600 nm
SMILES string   [Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1
InChI   1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChI key   IVGPGQSSDLDOLH-UHFFFAOYSA-M

Description

Application

Resazurin sodium salt has been used in viability assay:
• to measure cell viability of A. fumigatus
• to assess cell proliferation
• to determine A. fumigatus viability in high-throughput screenings of microbial natural extracts

For the measurement of metabolic activity and proliferation of living cells. The bioreduction of the dye reduces the amount of the oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

Packaging

1, 5 g in glass bottle

Biochem/physiol Actions

Resazurin is a blue, non-fluorescent dye originally used to test milk/biological fluids for bacterial and yeast contamination. It is also useful in sperm viability and semen quality test. Resazurin is applicable in cytotoxicity determination.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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