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R8755 Sigma-Aldrich

RPMI-1640 Medium

Modified, with L-glutamine, without phenol red and sodium bicarbonate, powder, suitable for cell culture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Culture Media, Cell Signaling and Neuroscience,
Quality Level   200
form   powder
application(s)   cell culture | mammalian: suitable
components   phenol red: no
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
HEPES: no
storage temp.   2-8°C

Description

Application

RPMI-1640 Medium is used for the following applications:
• Used in cell culture medium: Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components
• Used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
• Used for cell isolation and culture
• Used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo Proliferation Assay
• Used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
• Used for the preparation of antifungal agents

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at clonal densities. Use this medium when working with stem cells or when growing cells at low densities. Also recommended for in vitro diagnostics use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Elispot assays: State-of-the-art tools for functional analysis of cellular immunology

Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qual...
Keywords: Absorption, Adsorption, Amplification, Apoptosis, Cancer, Clinical, Cryopreservation, Degradations, Deposition, Detergents, Diagnostic, Diffusion, Diseases, Enzyme activity, Enzyme-linked immunosorbent assay, Evaporation, Filtration, Flow cytometry, Gene expression, Immobilization, Indicators, Microscopy, PAGE, Phase transitions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Reductions, Titrations, Western blot

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture. The entire contents of each package should be used immediately after opening. Preparing m...

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

RPMI Media - RPMI-1640 Media

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system...
Keywords: Vitamins

Peer-Reviewed Papers
15

References

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