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R8758 Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Biology, Cell Culture, Cell Culture Media, Classic Media and Salts, Classical Media,
Quality Level   PREMIUM
sterility   sterile-filtered
form   liquid
impurities   endotoxin, tested
suitability   suitable for cell culture
components   sodium pyruvate: no
L-glutamine: yes
phenol red: yes
HEPES: no
NaHCO3: yes
storage temp.   2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

RPMI-1640 Medium has been used for maintaining cell line medium and serves as a medium for different cells.

Linkage

Need FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
DoesProduct R8758, RPMI-1640 Medium, contain glucose?
RPMI contains glucose at 2 g/L. The complete formulation of RPMI and other classical media formulations can be found on the Cell Culture Web Page.
Can I add the glutamine dipeptide to Product R8758, RPMI-1640 Medium?
This formulation of RPMI already contains L-glutamine at 0.3 g/L. It does not require further supplementation with L-glutamine or the L-glutamine dipeptide (Product No. G8541).
Does Product R8758, RPMI-1640 Medium, contain HEPES?
There is no HEPES in this formulation of RPMI. We do offer RPMI containing HEPES as Product No's. R5886 and R4130. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
Can Product R8758, RPMI-1640 Medium, be frozen?
We do not recommend freezing cell culture media. A precipitate may form upon thawing.Also, this can cause a loss of amino acids, which may yield low cell growth.
Does Product R8758, RPMI-1640 Medium, contain phenol red?
This media contains phenol red. The formulation of RPMI and other classical media can be found on the Cell Culture Web Page.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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Cell Culture Guide
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Peer-Reviewed Papers
15

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