• USA Home
  • RAB0005 - Human ADIPOQ / Adiponectin ELISA Kit

EMAIL THIS PAGE TO A FRIEND
RAB0005 Sigma-Aldrich

Human ADIPOQ / Adiponectin ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym: 30 kDa adipocyte complement-related protein, ACDC, ACRP30, ADPN, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein

Purchase

Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type urine
sample type serum
sample type cell culture supernatant(s)
sample type plasma
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 24.69-18000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 25 pg/mL
standard curve range: 24.69-18000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ADIPOQ(9370)

Description

General description

The Human Adiponectin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Adiponectin in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human Adiponectin

Application

For research use only. Not for use in diagnostic procedures.
Human ADIPOQ / Adiponectin ELISA Kit has been used for the measurement of adiponectin in human plasma.

Biochem/physiol Actions

Adiponectin (ADIPOQ) is an adipocyte specific secreted protein that circulates in the plasma. The protein has a carboxyl-terminal globular domain and an amino-terminal collagen domain. It has anti-atherosclerotic and anti-inflammatory properties. ADIPOQ has a role in physiological processes such as energy homeostasis and obesity. It exhibits anti-diabetic action by interacting with adiponectin receptors, AdipoR1 and AdipoR2. Low levels of adiponectin are also linked with hypertension and dyslipidemia. Mutation in the gene encoding it is linked with risk for endometrial cancer.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

Human ADIPOQ / Adiponectin Antibody-coated ELISA Plate (Item A)    
Lyophilized Human ADIPOQ / Adiponectin Protein Standard (Item C)    
Biotinylated Human ADIPOQ / Adiponectin Detection Antibody (Item F)    
ELISA 1X Assay/Sample Diluent Buffer A (Item D1)    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)    
ELISA 1X Assay/Sample Diluent Buffer C (Item L)    
HRP-Streptavidin (Item G)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?