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RAB0026 Sigma-Aldrich

Human BDNF ELISA Kit

for serum, plasma, cell culture supernatant and urine

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type urine
sample type serum
sample type plasma
sample type cell culture supernatant(s)
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.066-16 ng/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... BDNF(627)

Description

General description

The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine.

The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.

Immunogen

Recombinant Human BDNF

Application

For research use only. Not for use in diagnostic procedures.
Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats. It has also been used to determine the plasma BDNF levels in GD1 (Gaucher’s disease type 1) patients and control population.

Human BDNF ELISA Kit has been used:
• to measure the brain-derived neurotrophic factor (BDNF) protein expression
• to determine BDNF level in the slice culture medium and the extracts of organotypic brain slice cultures (OBSCs)
• to determine BDNF level in plasma

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions. BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB). It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

Add to Cart

ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS RABELADA
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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