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  • RAB0036 - Human Carbonic Anhydrase IX ELISA Kit

RAB0036 Sigma-Aldrich

Human Carbonic Anhydrase IX ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym: CA9

  •  NACRES NA.32



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type urine
sample type cell culture supernatant(s)
sample type plasma
sample type serum
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.2 pg/mL
standard curve range: 0.205-50 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CA9(768)


General description

Carbonic anhydrase IX (CA9) belongs to the zinc-containing metalloenzymes family. The protein is encoded by the gene mapped to human chromosome 9p. The encoded protein is localized on cell membrane and is characterized with an N-terminal proteoglycan-like domain (PG), a CA catalytic domain (CA), a transmembrane helical segment and a short intra-cytoplasmic tail. CA9 is expressed at very low levels in normal tissues (mostly in gastrointestinal tract), but it is expressed at high level in various cancer tissues, such as renal, lung, cervical, ovarian, esophageal and breast cancer.

The Human CA-IX (Carbonic Anhydrase IX) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-IX in serum, plasma, cell culture supernatants and urine.


Recombinant Human CA9


For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Carbonic anhydrase IX (CA9) aids in reversible hydration of carbon dioxide to carbonic acid and bicarbonate. CA9 functions as a promising biomarker for diagnosing clear cell renal cell carcinoma (ccRCC), tumor hypoxia and prostate cancer (PC). In addition to pH control, CA9 also plays a vital role in cell proliferation, adhesion and tumor invasion.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately


Product #

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ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA 1X Assay/Sample Diluent Buffer C (Item L) SDS RABELADC
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
UN 3264 8 / PGIII


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

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ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
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