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RAB0058 Sigma-Aldrich

Rat MCP-1 / CCL2 ELISA Kit

for cell and tissue lysates

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   rat
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
assay range   inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 15 pg/mL
standard curve range: 24.67-18000 pg/mL
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... Ccl2(24770)

Description

General description

The Rat MCP-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat MCP-1 in cell lysate and tissue lysate.

Immunogen

Recombinant Rat MCP1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Chemokine (C-C motif) ligand 2 (CCL2) or monocyte chemoattractant protein-1 (MCP-1) is a pro-inflammatory factor expressed in astrocytes. It is synthesized by monocytes, B cells, fibroblast cells, endothelial and glial cells. CCL2 activates monocytes and regulates cytokine and adhesion molecule synthesis. The protein modulates neuropeptide function and development.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

ELISA 5X Sample Diluent Buffer (Item D2)    
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)    
HRP-Streptavidin (Item G)    
2X Cell Lysis Buffer (Item J)    
Rat MCP-1 / CCL2 Antibody-coated ELISA Plate (Item A)    
Biotinylated Rat MCP-1 / CCL2 Detection Antibody (Item F)    
Lyophilized Rat MCP-1 / CCL2 Protein Standard (Item C)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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