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RAB0093 Sigma-Aldrich

Human CNTF ELISA Kit

for serum, plasma, cell culture supernatant and urine

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type urine
sample type cell culture supernatant(s)
sample type serum
sample type plasma
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 200 pg/mL
standard curve range: 0.823-200 ng/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CNTF(1270)

Description

General description

The Human CNTF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CNTF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CNTF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Ciliary neurotrophic factor (CNTF) is part of the cytokine family. It signals through the glycoprotein 130 and LIF receptor β subunit. The protein has neuroprotective effects and activates astrocytes. CNTF also has a role in the differentiation of neural stem cells and is involved in neurotransmitter synthesis.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

Add to Cart

ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS RABELADA
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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