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RAB0147 Sigma-Aldrich

Human DPPIV / CD26  ELISA Kit

for serum, plasma, cell culture supernatant and urine

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type urine
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 32.77-8000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... DPP4(1803)

Description

General description

The Human CD26 (also known as DPPIV) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CD26 in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human CD26

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Dipeptidyl peptidase 4 (DPPIV), also known as CD26 (cluster of differentiation 26), is localized on T- cell surface and interacts with adenosine deaminase (ADA), collagen and CD459. It plays a vital role in T cell co-stimulation, cell-to-cell adhesion, and in human immunodeficiency virus (HIV) infection. Deletion of the DPPIV gene alters carbohydrate metabolism which in turn leads to mental retardation and hypotonia. Abnormal expression of DPPIV is observed during liver regeneration, human cirrhosis and liver tumorigenesis. DPPIV level is reduced in serum of chronic obstructive pulmonary disease (COPD) patients, hence, this protein has potential as a serologic marker for screening COPD. DPP4 functions as a serine protease enzyme that catalyzes the cleavage of dipeptides from peptides containing proline or alanine residues. IL-13 induced DPPIV expression is upregulated in bronchial epithelial cells (BECs) and this expression might contribute to the development of asthmatic airway inflammation, cell proliferation and fibronectin (FN) production. This protein is a promising biomarker and therapeutic target for asthma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

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ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS RABELADA
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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