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  • RAB0182 - Human bFGF ELISA Kit

RAB0182 Sigma-Aldrich

Human bFGF ELISA Kit

for serum, plasma, cell culture supernatant and urine

  •  NACRES NA.32



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 102.4-10000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... FGF2(2247)


General description

The Human bFGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human bFGF in serum, plasma, cell culture supernatants and urine.


Recombinant Human basic-FGF


Human bFGF ELISA kit has been used:
• to quantify the level of residual cytokines in decellularized meningeal scaffolds (DMS)
• to measure cytokine levels in acellularized meningeal scaffolds (AMS) and recovered meningeal tissues (RMS/CMS)
• to analyze basic-fibroblast growth factor (bFGF) concentration in cell culture medium

Biochem/physiol Actions

FGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3. It stimulates the proliferation of a wide variety of cells, including mesenchymal, neuroectodermal and endothelial cells. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It plays an important role in spinal cord injury repair. FGF2 is known to stimulate angiogenesis and is associated with cancer development. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. MicroRNA (miR)-503 targets FGF2 mRNA and inhibits migration and invasiveness of osteosarcoma (OS) cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately


Product #

Add to Cart

ELISA 5X Sample Diluent Buffer (Item D2) SDS RABDIL6
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
UN 3264 8 / PGIII


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
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