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RAB0273 Sigma-Aldrich

Human IL-1 β ELISA Kit

for serum, plasma, cell culture supernatant and urine

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.3 pg/mL
standard curve range: 0.48-100 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 0.3 pg/mL
standard curve range: 0.48-100 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IL1B(3553)

Description

General description

The Human IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 β in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human IL-1β

Application

For research use only. Not for use in diagnostic procedures.
Human IL-1 β ELISA kit has been used to measure the concentration of interleukin 1β (IL-1 β) in cell culture supernatants.

Biochem/physiol Actions

Interleukin 1β (IL-1 β) is a proinflammatory cytokine, which is implicated in induction of various acute and chronic inflammation. Hence, IL1B can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β protein increases the production of endogenous 92kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). Elevated concentration of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. IL-1β brings neutrophil and macrophage to the site of infection. Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura (HSP).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

Human IL-1 β Antibody-coated ELISA Plate (Item A)    
Lyophilized Human IL-1 β Protein Standard (Item C)    
Biotinylated Human IL-1 β Detection Antibody (Item F)    
ELISA 1X Assay/Sample Diluent Buffer A (Item D1)    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)    
HRP-Streptavidin (Item G)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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