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RAB0274 Sigma-Aldrich

Mouse IL-1 β ELISA Kit

for serum, plasma and cell culture supernatant

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   mouse
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type serum
sample type plasma
sample type cell culture supernatant(s)
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Il1b(16176)

Description

General description

The Mouse IL-1β ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1β in serum, plasma, cell culture supernatants and urine. Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa. IL-1β is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

Immunogen

Recombinant Mouse IL-1 β

Application

Mouse IL-1 β ELISA Kit has been used to determine the presence of interleukin-1β (IL-1β) in plasma sample. It has also been used to determine the serum concentration levels of IL-1β.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

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ELISA 1X Assay/Sample Diluent Buffer A (Item D1)    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)    
HRP-Streptavidin (Item G)    
Mouse IL-1 β Antibody-coated ELISA Plate (Item A)    
Biotinylated Mouse IL-1 β Detection Antibody (Item F)    
Lyophilized Mouse IL-1 β Protein Standard (Item C)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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