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RAB0275 Sigma-Aldrich

Mouse IL-1 β ELISA Kit

for cell and tissue lysates

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   mouse
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type cell lysate
sample type tissue lysate
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Il1b(16176)

Description

General description

The Mouse IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 β cell lysate and tissue lysate.

Immunogen

Recombinant Mouse IL-1 β

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse IL-1 β ELISA Kit has been used to measure the concentration of interleukin 1 (IL)1-β in serum and bronchoalveolar lavage fluid (BALF) sample.

Biochem/physiol Actions

Interleukin 1 β (IL-1β) stimulates the production of endogenous 92 kDa gelatinase (matrix metalloproteinases 9 (MMP-9)) and tissue inhibitors of MMP (TIMP -1). It is a proinflammatory cytokine that induces various acute and chronic inflammation. Therefore, IL-1β can be considered as a potential therapeutic target for disease associated with fibrosis and tissue remodeling. IL-1β brings neutrophil and macrophage to the site of infection. Over expression of IL-1β in synovial fluid contributes to the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joint. Mutations in IL-1β gene leads to renal manifestations and renal sequelae in Henoch-Schonlein purpura (HSP).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

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ELISA 5X Sample Diluent Buffer (Item D2)    
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)    
HRP-Streptavidin (Item G)    
Mouse IL-1 β Antibody-coated ELISA Plate (Item A)    
Biotinylated Mouse IL-1 β Detection Antibody (Item F)    
Lyophilized Mouse IL-1 β Protein Standard (Item C)    
2X Cell Lysis Buffer (Item J)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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