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RAB0277 Sigma-Aldrich

Rat IL-1 β ELISA Kit

for serum, plasma and cell culture supernatant

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   rat
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type plasma
sample type cell culture supernatant(s)
sample type serum
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... Il1b(24494)

Description

General description

The Rat IL-1 β ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1 β in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Rat IL-1 β

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

ELISA 1X Assay/Sample Diluent Buffer A (Item D1)    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)    
HRP-Streptavidin (Item G)    
Rat IL-1 β Antibody-coated ELISA Plate (Item A)    
Biotinylated Rat IL-1 β Detection Antibody (Item F)    
Lyophilized Rat IL-1 β Protein Standard (Item C)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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Technical Service:

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