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  • RAB0278 - Rat IL-1 β ELISA Kit

RAB0278 Sigma-Aldrich

Rat IL-1 β ELISA Kit

for cell and tissue lysates



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   rat
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type cell lysate
sample type tissue lysate
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... Il1b(24494)


General description

The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate.


Recombinant Rat IL-1 β


For research use only. Not for use in diagnostic procedures

Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels .
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only


Product #

Add to Cart

ELISA 5X Sample Diluent Buffer (Item D2)    
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)    
HRP-Streptavidin (Item G)    
2X Cell Lysis Buffer (Item J)    
Rat IL-1 β Antibody-coated ELISA Plate (Item A)    
Biotinylated Rat IL-1 β Detection Antibody (Item F)    
Lyophilized Rat IL-1 β Protein Standard (Item C)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
UN 3264 8 / PGIII


Certificate of Analysis

Protocols & Articles


Antibody Basics

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ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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