• USA Home
  • RAB0278 - Rat IL-1 β ELISA Kit

EMAIL THIS PAGE TO A FRIEND
RAB0278 Sigma-Aldrich

Rat IL-1 β ELISA Kit

for cell and tissue lysates

Purchase

Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   rat
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type cell lysate
sample type tissue lysate
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
  inter-assay cv: <12 %
intra-assay cv: <10 %
sensitivity: 80 pg/mL
standard curve range: 68.59-50000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   rat ... Il1b(24494)

Description

General description

The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate.

Immunogen

Recombinant Rat IL-1 β

Application

For research use only. Not for use in diagnostic procedures

Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels .
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

ELISA 5X Sample Diluent Buffer (Item D2)    
ELISA 5X Assay/Sample Diluent Buffer E (Item E2)    
HRP-Streptavidin (Item G)    
2X Cell Lysis Buffer (Item J)    
Rat IL-1 β Antibody-coated ELISA Plate (Item A)    
Biotinylated Rat IL-1 β Detection Antibody (Item F)    
Lyophilized Rat IL-1 β Protein Standard (Item C)    
ELISA Stop Solution (Item I)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?