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RAB0326 Sigma-Aldrich

C-Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, EIA Kits, ELISA Kits
species reactivity   human, rat, mouse
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  enzyme immunoassay: suitable
input   sample type cell lysate
sample type culture supernatant(s)
sample type plasma
sample type serum
assay range   inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 772 pg/mL
standard curve range: 0.1-1000 ng/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... INS(3630)

Description

General description

The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.

Immunogen

C-Peptide , synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

C-peptide inhibits endothelial dysfunction under high glucose conditions via inhibition of nuclear factor-κB (NF-κB) pathway. Experimental studies suggest that prolonged dosage of physiological concentration of C-peptide to type 1 diabetes patients minimizes diabetes-induced complications, such as glomerular hypertrophy and mesangial matrix expansion by interrupting transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNFα) signaling pathway. C-peptide reduces diabetes-induced glomerular hyperfiltration by contraction of the afferent arteriole, dilation of the efferent arteriole, and by inhibition of tubular reabsorption. C-Peptide also plays a crucial role in renal pathophysiology. C-peptide binds to a cell surface receptor that is likely to be G-protein coupled. This interaction stimulates various cellular functions such as increasing intracellular Ca2+ concentration, enhancing PI3-K (phosphoinositide 3-kinase) activity, increased endothelial nitric oxide synthase (eNOS) transcription, and activation of the Na+/K+ ATPase and mitogen-activated protein kinase (MAPK) signaling pathway.

Kit component also available separately

Description

Product #

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HRP-Streptavidin SDS RABHRP3
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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