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RAB0333 Sigma

Human Leptin ELISA Kit

for serum, plasma, cell culture supernatant and urine



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
application(s)   ELISA: suitable
  capture ELISA: suitable
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... LEP(3952)



Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

General description

The Human Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Leptin in serum, plasma, cell culture supernatants and urine.


Recombinant Human Leptin

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Price and Availability

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Human Leptin Antibody-coated ELISA Plate (Item A) SDS    
Lyophilized Human Leptin Protein Standard (Item C) SDS    
Biotinylated Human Leptin Detection Antibody (Item F) SDS    
ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS    
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS    
HRP-Streptavidin (Item G) SDS    
ELISA Stop Solution (Item I) SDS    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS    
20X Wash Buffer (Item B) SDS    
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


ELISA Protocols

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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