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RAB0334 Sigma-Aldrich

Mouse Leptin ELISA Kit

for serum, plasma, cell culture supernatant

Synonym: OB

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   mouse
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type cell culture supernatant(s)
sample type plasma
sample type serum
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 4 pg/mL
standard curve range: 4.1-1000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Lep(16846)

Description

General description

The Mouse Leptin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Leptin in serum, plasma and cell culture supernatants.

Immunogen

Recombinant Mouse Leptin

Application

For research use only. Not for use in diagnostic procedures.

Mouse Leptin ELISA Kit has been used to measure plasma leptin concentration.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse Leptin ELISA kit has been used to measure serum leptin concentration in adipose tissues of mice.

Biochem/physiol Actions

Leptin is a hormone secreted by adipocytes. It is majorly involved in regulation of food intake and energy balance. Variation in the leptin promoter alters serum leptin and HDLC concentration, which might lead to development of obesity. Increased concentration of serum leptin causes arterial stiffness in coronary artery disease (CAD) patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

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ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS RABELADA
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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